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her time is over...They made a flashback just for the anime...
No, I don't think Lisana will appear again. Though it would have been great if she came back to life.
Though, she is a precious part of the guild as a memory.
If Mashima wish to bring her back he will.
Gerard/Jaleel is proof of that.
Lisana on the other had died in a manner that left a body judging by Mirajane's flashback. As such, no one was in doubt that she's dead.
My arguement is. If Mashima wanted. He could bring her back in any way, shape or form. Don't forget, this manga is about "Magic".